bcl2 selective inhibitor abt 199 (MedChemExpress)
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Bcl2 Selective Inhibitor Abt 199, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 251 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 97 stars, based on 251 article reviews
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Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer.
Journal: Cancer medicine
doi: 10.1002/cam4.2440
Figure Legend Snippet: FIGURE 1 HOXA10 and BCL2 expressions were elevated in GC tissues. A and B, qRT‐PCR and western blot indicated that the expression of HOXA10 was higher in GC tissues (qRT‐PCR values were arranged from low to high). C, HOXA10 was elevated in the STAD reanalyzed from GEPIA. D, HOXA10 ranked fourth of the top 25 overexpressed genes in the STAD from UALCAN. E, Higher expression of HOXA10, BCL2 demonstrated lower overall survival rate in GC patients reanalyzed from the Kaplan‐Meier Plotter. F, Representative immunohistochemical staining showed that HOXA10, BCL2, and Ki67 were upregulated in GC tissues. Original magnification, 100× (200× for insert images). GC, gastric cancer; qRT‐PCR, quantitative real‐time polymerase chain reaction; STAD, stomach adenocarcinoma dataset
Techniques Used: Quantitative RT-PCR, Western Blot, Expressing, Immunohistochemical staining, Staining, Real-time Polymerase Chain Reaction
Figure Legend Snippet: FIGURE 2 HOXA10 promoted proliferation and repressed apoptosis in GC cells. A and B, The CCK‐8 assay showed that HOXA10 knockdown inhibited cell growth but could be partly rescued by transfecting with BCL2‐overexpressing plasmid. And, HOXA10 overexpression promoted cell growth but could be partially impaired with treatment of BCL2 selective inhibitor ABT‐199. C and D, HOXA10 knockdown inhibited colony formation in BGC‐823 cells while HOXA10 overexpression enhanced colony formation in AGS and HGC‐27 cells. E and F, The cell apoptosis assay showed the percentage of apoptotic cells was higher in HOXA10‐knockdown cells (BGC‐823‐sh‐HOXA10, NCI‐N87‐ sh‐HOXA10) compared with the control cells, but could be partly reduced by transfecting with BCL2‐overexpressing plasmid. And, the portion of apoptotic cells was lower in HOXA10‐overexpressinig cells (AGS‐HOXA10‐OV, HGC‐27‐HOXA10‐OV) compared with the corresponding control cells, but could be partially rescued with treatment of ABT‐199. **P < .01, ***P < .001. CCK‐8, cell counting kit‐8. GC, gastric cancer
Techniques Used: CCK-8 Assay, Knockdown, Plasmid Preparation, Over Expression, Apoptosis Assay, Control, Cell Counting
Figure Legend Snippet: FIGURE 3 HOXA10 promoted tumor growth in the nude mice xenograft tumor formation assay. A, Tumors formed by AGS‐HOXA10‐OV cells demonstrated an elevation of bioluminescent signal. B‐D, The weight and volume of tumors formed by AGS cells in different groups. HOXA10 overexpression promoted xenograft tumor growth in nude mice. E‐H, The weight and volume of tumors formed by NCI‐N87 cells showed HOXA10 knockdown inhibited xenograft tumor growth in nude mice. I and J, Representative immunohistochemical staining of HOXA10, BCL2, and Ki67 in xenograft tumors. Original magnification, 200×. *P < .05
Techniques Used: Tube Formation Assay, Over Expression, Knockdown, Immunohistochemical staining, Staining
Figure Legend Snippet: FIGURE 4 The bioinformatics analysis, qRT‐PCR, western blot, and ChIP‐qPCR assay revealed that HOXA10 might induce BCL2 expression via binding to its promoter region. A, The “TF‐gene interactions” analysis of HOXA10 and BCL2 in the database NetworkAnalyst. B, A PPI network was built between HOXA10 and BCL2 with the database STRING. C, qRT‐PCR showed the relative BCL2 mRNA level in different cells with changed HOXA10 expression. D, Expression of BCL2, Bax, cleaved forms of Caspase‐9, Caspase‐3, and PARP in different GC cells with altered HOXA10 expression. E, HOXA10 binding motif acquired from JASPAR and the relative primer position within the BCL2 promoter region. F, ChIP‐qPCR assay indicated the possible positive HOXA10 binding sites across the BCL2 promoter region. G, 3% agarose gel electrophoresis of ChIP‐qPCR products. **P < .01, ***P < .001. ChIP‐qPCR, chromatin immunoprecipitation and quantitative PCR; PPI, protein‐ protein interaction; qRT‐PCR, quantitative real‐time polymerase chain reaction; TF‐gene, transcription factor‐gene
Techniques Used: Quantitative RT-PCR, Western Blot, ChIP-qPCR, Expressing, Binding Assay, Agarose Gel Electrophoresis, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction
Related Articles
Quantitative RT-PCR:Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer. Article Snippet: BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).. The Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer Article Snippet: represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen). .. The Western Blot:Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer. Article Snippet: BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).. The Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer Article Snippet: represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen). .. The Expressing:Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer. Article Snippet: BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).. The Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer Article Snippet: represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen). .. The Immunohistochemical staining:Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer. Article Snippet: BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).. The Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer Article Snippet: represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen). .. The Staining:Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer. Article Snippet: BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).. The Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer Article Snippet: represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen). .. The Real-time Polymerase Chain Reaction:Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer. Article Snippet: BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).. The Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer Article Snippet: represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen). .. The CCK-8 Assay:Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer. Article Snippet: BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).. The Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer Article Snippet: represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen). .. The Knockdown:Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer. Article Snippet: BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).. The Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer Article Snippet: represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen). .. The Plasmid Preparation:Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer. Article Snippet: BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).. The Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer Article Snippet: represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen). .. The Over Expression:Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer. Article Snippet: BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).. The Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer Article Snippet: represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen). .. The Apoptosis Assay:Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer. Article Snippet: BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).. The Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer Article Snippet: represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen). .. The Control:Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer. Article Snippet: BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).. The Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer Article Snippet: represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen). .. The Cell Counting:Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer. Article Snippet: BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).. The Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer Article Snippet: represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen). .. The Tube Formation Assay:Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer. Article Snippet: BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).. The Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer Article Snippet: represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen). .. The ChIP-qPCR:Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer. Article Snippet: BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).. The Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer Article Snippet: represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen). .. The Binding Assay:Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer. Article Snippet: BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).. The Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer Article Snippet: represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen). .. The Agarose Gel Electrophoresis:Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer. Article Snippet: BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).. The Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer Article Snippet: represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen). .. The Chromatin Immunoprecipitation:Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer. Article Snippet: BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).BGC‐823‐sh‐ HOXA10+BCL2 or NCI‐N87‐sh‐HOXA10+BCL2 represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen).. The Article Title: HOXA10 induces BCL2 expression, inhibits apoptosis, and promotes cell proliferation in gastric cancer Article Snippet: represented BGC‐823‐sh‐HOXA10 or NCI‐N87‐sh‐HOXA10 cells transfected with BCL2‐overexpressing plasmid using Lipofectamine 2000 (Invitrogen). .. The |